Scheme 1
From: In vitro detection of cancer cells using a novel fluorescent choline derivative
Synthesis of 1. Reagents and conditions: [a] 2-((4-aminophenyl)(ethyl)amino)ethanol sulfate (2 equiv), DABCO (4 equiv), decahydronaphthalene, 175 °C, 72 h, N2; [b] CBr4 (1.2 equiv), PPh3 (1.2 equiv), CH2Cl2, 0 °C to rt, 16 h, N2; [c] 2-(dimethylamino)ethanol (1.5 equiv), THF, 50 °C, 72 h, N2